黑龙江医学2024，Vol.48Issue(23) ：2848-2850.DOI:10.3969/j.issn.1004-5775.2024.23.009

ADAM8的N-糖基化突变(N436Q)对小鼠胶质母细胞瘤的影响

Effect of N-glycosylation Mutation(N436Q)of ADAM8 on Glioblastoma in Mice

杨建权 王国兵 张钶 戴永建 王志涛 阿尔帕提·买买提 关玉华 杨晓玲 王增亮
黑龙江医学2024，Vol.48Issue(23) ：2848-2850.DOI:10.3969/j.issn.1004-5775.2024.23.009
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  • 维普
  • 万方数据

ADAM8的N-糖基化突变(N436Q)对小鼠胶质母细胞瘤的影响

Effect of N-glycosylation Mutation(N436Q)of ADAM8 on Glioblastoma in Mice

杨建权 1王国兵 2张钶 2戴永建 2王志涛 1阿尔帕提·买买提 1关玉华 3杨晓玲 3王增亮1
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作者信息

  • 1. 新疆医科大学第一附属医院神经外科,新疆 乌鲁木齐 830054
  • 2. 湖北医药学院附属人民医院神经外科,湖北 十堰 442000
  • 3. 巴音郭楞蒙古自治州人民医院,新疆 库尔勒 841000
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摘要

目的:探讨解整合素金属蛋白酶8(ADAM8)的N-糖基化对肿瘤生长和上皮间质转化(EMT)的影响.方法:通过定点突变技术将ADAM8的N-糖基化位点N436突变(N436Q)为谷氨酰胺,并在稳定转染的U87细胞中表达这一突变体.将这些细胞植入BALB/c裸鼠中,评估肿瘤生长情况.结果:ADAM8的N-糖基化缺陷突变体抑制了肿瘤的生长、减小了肿瘤体积和重量.进一步分析发现,ADAM8的N-糖基化缺陷突变体下调了EMT相关蛋白Vimentin和Claudin-1的表达并上调了E-cadherin的表达.结论:抑制ADAM8的N-糖基化可抑制小鼠胶质瘤的生长和EMT过程.

Abstract

Objective:To investigate the effects of N-glycosylation of A Disintegrin and Metalloproteinase 8(ADAM8)on tumor growth and epithelial mesenchymal transformation(EMT).Methods:The N-glycosylation site N436Q of ADAM8 was mutated to glutamine with site-specific mutation technique,and the mutant was expressed in stably transfected U87 cells.These cells were implanted in BALB/c nude mice,and tumor growth was assessed.Results:ADAM8 N-glycosylation defect mutant inhibited tumor growth and reduced tumor volume and weight.Further analysis showed that ADAM8 N-glycosylation deficient mutant down-regulated the expression of EMT-related proteins Vimentin and Claudin-1,and up-regulated the expression of E-cadherin.Conclusion:Inhibition of ADAM8 N-glycosylation can inhibit the growth of glioblastoma in mice by inhibiting EMT process.

关键词

解整合素金属蛋白酶8/N-糖基化/上皮间质转化/胶质母细胞瘤

Key words

ADAM8/N-glycosylation/Epithelial interstitial transition/Glioblastoma

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出版年

2024
黑龙江医学
中华医学会黑龙江分会

黑龙江医学

影响因子：0.714
ISSN：1004-5775
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