Effect of N-glycosylation Mutation(N436Q)of ADAM8 on Glioblastoma in Mice
Objective:To investigate the effects of N-glycosylation of A Disintegrin and Metalloproteinase 8(ADAM8)on tumor growth and epithelial mesenchymal transformation(EMT).Methods:The N-glycosylation site N436Q of ADAM8 was mutated to glutamine with site-specific mutation technique,and the mutant was expressed in stably transfected U87 cells.These cells were implanted in BALB/c nude mice,and tumor growth was assessed.Results:ADAM8 N-glycosylation defect mutant inhibited tumor growth and reduced tumor volume and weight.Further analysis showed that ADAM8 N-glycosylation deficient mutant down-regulated the expression of EMT-related proteins Vimentin and Claudin-1,and up-regulated the expression of E-cadherin.Conclusion:Inhibition of ADAM8 N-glycosylation can inhibit the growth of glioblastoma in mice by inhibiting EMT process.
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维普
