Objective:To investigate the value of p16 /Ki-67 double staining and HPVE6 /E7mRNA detection in the diagnosis of cervical lesions.Methods:605 high-risk HPV-positive patients admitted to our hospital from April 2021 to February 2023 were selected as the study objects.Colposcopy and pathological biopsy were performed on all patients after admission,and cytological samples were collected for p16 /Ki-67 double staining and HPVE6 /E7mRNA detection,respectively.The positive detection rates of cervical lesions were compared between the two methods.To compare the efficacy of three methods in the diagnosis of cervical lesions. Results:The positive rates of CIN1-3 detected by combined assay were higher than those detected by p16 /Ki-67 double labeling and HPVE6 /E7mRNA (P<0.05).There was no significant difference in the positive detection rate of invasive carcinoma among the three methods (P>0.05).The results of Kappa test showed that the results of p16 /Ki-67 double labeling and HPVE6 /E7mRNA were consistent with the pathological diagnosis results (Kappa value =0.719,P<0.05).The results of combined detection were highly consistent with those of pathological diagnosis (Kappa =0.894,P<0.05).The diagnostic sensitivity,specificity and accuracy of combined detection for cervical lesions were higher than p16 /Ki-67 double labeling and HPVE6 /E7mRNA detection (P<0.05). Conclusion:The combined detection of p16Ki-67 double staining and HPVE6 /E7mRNA can accurately detect the degree of cervical lesions,and provide sufficient and reliable data support for the identification of cervical lesions and disease types.The combined detection has good diagnostic efficacy,and has positive guiding significance for the follow-up treatment of patients.
维普
万方数据
