Objective:To establish an organic-free extraction method by using surfactants combined with ultrasonic-assisted extraction of camptothecin from Camptotheca acuminata seeds.Methods:The surfactant was screened and its optimal concentration was determined by the solubility of surfactant in camptothecin.The single factor experiment and orthogonal experiment were conducted on the liquid-solid ratio,ultrasonic power,ultrasonic time and temperature in the extraction process.Results:The optimal extraction conditions for camptothecin with 13％PEG400 aqueous solution were liquid-solid ratio of 90:1,ultrasonic power of 540W,ultrasonic time of 90min and temperature of 25℃.Under these conditions,the extraction rate of camptothecin was 1174.59 pg/g.Conclusion:Camptothecin was successfully extracted from Camptotheca acuminata seeds with PEG400 aqueous solution,and the extraction rate was 1174.59 µg/g.
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