首页|牙周炎患者唾液微生物对抑郁大鼠行为和炎症因子的影响

牙周炎患者唾液微生物对抑郁大鼠行为和炎症因子的影响

扫码查看
目的:探讨牙周炎患者唾液微生物对抑郁大鼠行为和炎症因子的影响.方法:使用慢性不可预知轻度应激法(Chronic unpredictable mild stress,CUMS)构建抑郁大鼠模型.造模结束后,对照组和CUMS组磷酸缓冲盐溶液(Phosphate buffered saline,PBS)PBS 灌胃,H-CUMS 组灌胃 30 名健康个体的唾液微生物(healthy salivary microbiota,HSM),P-CUMS 组灌胃30名牙周炎患者的唾液微生物(periodontitis salivary microbiota,PSM),每隔一天灌胃1次,共2周.灌胃结束后对所有大鼠进行旷场实验和强迫游泳实验测试,取外周血血清,ELISA方法检测炎症因子IL-6和TNF-a的含量.结果:P-CUMS组的运动距离减少(P=0.022),平均速度减慢(P=0.019),绝望行为增加(P=0.030),外周血中IL-6(P=0.015)和TNF-a(P=0.048)含量明显升高,与H-CUMS组相比均有统计学差异.结论:牙周炎患者的唾液微生物加剧了抑郁大鼠的抑郁样行为和炎症反应,预防牙周病的发生,对MDD的治疗和预后起重要作用.
Effects of Saliva Microbiota in Patients with Periodontitis on Behavior and Inflammatory Factors in Depressed Rats
Objective:To investigate the effects of salivary microbiota in periodontitis patients on behavior and inflammatory factors in depressed rats.Methods:Chronic unpredictable mild stress(CUMS)was used to construct a depressed rat model.After molding,Control group and CUMS group were administrated with PBS.H-CUMS group was administrated with healthy salivary microbiota(HSM)from 30 healthy individuals,and P-CUMS group was administrated with periodontitis salivary microbiota(PSM)from 30 patients with periodontitis,once every other day.After 2 weeks of gavage,all the rats were subjected to open field experiment and forced swimming experiment to test their depression-like behavior.The levels of inflammatory factors IL-6 and TNF-a in rat peripheral blood serum were detected by ELISA.Results:Compared with the Control group,the CUMS group had reduced motor distance(P=0.022),slowed down average speed(P=0.019),increased despair behavior(P=0.030),and increased the contents of IL-6(P=0.015)and TNF-a(P=0.048)in peripheral blood.There were statistical differences in all the above.Conclusion:Saliva microbiota from periodontitis patients exacerbated depression-like behavior and inflammatory responses in depressed rats.The prevention of periodontal disease may be play an important role in the treatment and prognosis of MDD.

PeriodontitisMDDDepression-like behaviorInflammatory factors

李琰、张宇涛、闫倩

展开 >

山西医科大学汾阳学院口腔教研室(山西 吕梁 032200)

牙周炎唾液微生物 MDD 抑郁样行为 炎症因子

山西省高校科技创新计划项目

2023L477

2024

黑龙江医药
黑龙江省药品审评认证中心

黑龙江医药

影响因子:0.597
ISSN:1006-2882
年,卷(期):2024.37(2)
  • 15