Effects of Saliva Microbiota in Patients with Periodontitis on Behavior and Inflammatory Factors in Depressed Rats
Objective:To investigate the effects of salivary microbiota in periodontitis patients on behavior and inflammatory factors in depressed rats.Methods:Chronic unpredictable mild stress(CUMS)was used to construct a depressed rat model.After molding,Control group and CUMS group were administrated with PBS.H-CUMS group was administrated with healthy salivary microbiota(HSM)from 30 healthy individuals,and P-CUMS group was administrated with periodontitis salivary microbiota(PSM)from 30 patients with periodontitis,once every other day.After 2 weeks of gavage,all the rats were subjected to open field experiment and forced swimming experiment to test their depression-like behavior.The levels of inflammatory factors IL-6 and TNF-a in rat peripheral blood serum were detected by ELISA.Results:Compared with the Control group,the CUMS group had reduced motor distance(P=0.022),slowed down average speed(P=0.019),increased despair behavior(P=0.030),and increased the contents of IL-6(P=0.015)and TNF-a(P=0.048)in peripheral blood.There were statistical differences in all the above.Conclusion:Saliva microbiota from periodontitis patients exacerbated depression-like behavior and inflammatory responses in depressed rats.The prevention of periodontal disease may be play an important role in the treatment and prognosis of MDD.