Isolationand Identification of Camptothecin-Producing Endophytic Fungus Aspergillus Niger GP3 Isolated From Camptotheca acuminata
Objective:Strains with potential to produce camptothecin were screened from endophytic fungi of Camptotheca camptotheca.,with the aim to provide a source of camptothecin.Methods:Endophytic fungus from root bark of Camptotheca acuminata,was used to isolated,active component of endophytic fungi secondary metaboliteswas detected by HPLC and UPLC-Orbitrap-MS/MS.The specific fungi were identified by morphology and molecular analysis.Results:Endophyte GP3 is an endophytic fungus strain with potential to produce camptothecin.The colony is round,dark brown,with a raised center and a ciliated margin.The ITS sequence is 99％similar to the corresponding sequence of Aspergillus niger.Conclusion:A camptothecin-producing endophytic fungus GP3 was isolated from the Camptotheca acuminata and identified as Aspergillus niger.
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