喜树中一株特异产喜树碱内生真菌Aspergillus niger GP3的分离与鉴定
Isolationand Identification of Camptothecin-Producing Endophytic Fungus Aspergillus Niger GP3 Isolated From Camptotheca acuminata
魏巍 1高雪霞 1徐铖 1安昊 1屈保玉 1顾成波 1袁肖寒2
作者信息
- 1. 东北林业大学 化学化工与资源利用学院 森林植物生态学教育部重点实验室 林业生物制剂教育部工程研究中心黑龙江省林源活性物质生态利用重点实验室(哈尔滨 150040)
- 2. 东北农业大学生命科学学院(哈尔滨 150030)
- 折叠
摘要
目的:从喜树内生真菌中筛选出具有产喜树碱潜力的菌株,为获得喜树碱提供一条新的药源途径.方法:从喜树根皮分离内生真菌,综合利用高效液相色谱法(HPLC)以及超高效液相色谱-高分辨质谱(UPLC-Orbitrap-MS/MS)法对内生真菌次生代谢产物有效活性成分进行分析,并通过形态学和分子学对其特异性真菌进行鉴定.结果:内生真菌GP3具有产喜树碱的化学性质,其菌落圆形、黑褐色、中心凸起、边缘呈纤毛状,经ITS序列比对,该菌与Aspergillus niger ITS序列相似性为99％.结论:GP3是一株具有产喜树碱潜力的内生真菌菌株,鉴定为黑曲霉菌(Aspergillus niger).
Abstract
Objective:Strains with potential to produce camptothecin were screened from endophytic fungi of Camptotheca camptotheca.,with the aim to provide a source of camptothecin.Methods:Endophytic fungus from root bark of Camptotheca acuminata,was used to isolated,active component of endophytic fungi secondary metaboliteswas detected by HPLC and UPLC-Orbitrap-MS/MS.The specific fungi were identified by morphology and molecular analysis.Results:Endophyte GP3 is an endophytic fungus strain with potential to produce camptothecin.The colony is round,dark brown,with a raised center and a ciliated margin.The ITS sequence is 99％similar to the corresponding sequence of Aspergillus niger.Conclusion:A camptothecin-producing endophytic fungus GP3 was isolated from the Camptotheca acuminata and identified as Aspergillus niger.
关键词
喜树/内生真菌/分离/鉴定/喜树碱Key words
Camptotheca acuminata/Endophytic fungi/Isolation/Identification/Camptothecin引用本文复制引用
基金项目
国家重点研发计划子课题(十四五)(2022YFD2200805-03)
出版年
2024
NETL
NSTL
万方数据