Study on the Mechanism of Bufeixiaojie Decoction on Lung Cancer Rats Based on VEGF-VEGFR Signaling Pathway
Objective:To investigate the Vascular endothelial growth factor(VEGF)or VEGF receptor-2(Vascular endothelial growth factor receptor-2)based on Bufei Xiaojie Decoction.The specific mechanism of VEGFR-2 signaling pathway on lung cancer rats.Methods:In this study,30 cases of transplanted tumor model of A549 lung cancer rats were established and randomly divided into control group(n=30),Bufei Xiaojie Decoction low-dose group(n=30)and high-dose group(n=30).Bufei Xiaojie Decoction low-dose group and high-dose group were given gavage of 10mL/kg and 20mL/kg,respectively.The control group was given the same amount of normal saline intragastrically,once a day in each group,for 14 days.The lung tissue was observed by histopathology.Meanwhile,Quantitative real-time PCR(qRT-PCR)was used to detect p53 mRNA expression in lung cancer tissue,and Western blot was used to detect VEGF and VEGFR-2 expression in rat lung tissue.Results:Compared with control group,p53 mRNA level in low and high dose groups was significantly increased(P＜0.05).Compared with the control group,the expressions of VEGF and VEGFR-2 in low and high dose groups were significantly decreased(P＜0.05).Conclusion:Bufei Xiaojie Decoction can regulate the gene ex-pression of VEGF-VEGFR signaling pathway,increase the expression level of p53 mRNA,and inhibit the growth of lung cancer in rats.
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