Exploring the Mechanism of Sophora Flavescens Mediated NF-κB Pathway Against Hepatocellular Based on Target Network and Molecular Docking
Objective:The present study aimed to explore the mechanism of the effective active ingredients in Sophora flavescens in resisting hepatocellular carcinoma(HCC)through network pharmacology,molecular docking,vivo and vitro experiments.Methods:The effective active ingredients of Sophora flavescens and potential targets for the treatment of HCC from TCMSP Database,SwissTarget-Prediction Database,GeneCards and Online Mendelian Inheritancein Man(OMIM).The protein-protein interaction(PPI)network of the common targets was constructed by STRING,and the key targets were sorted and filtered out according to the node degree.GO and KEGG pathway enrichment analysis of key target genes were selected through the DAVID data platform.Through Discovery Studio,CASP3 and STAT3 were selected as the key targets for molecular docking with the first five active components.Finally,HepG2 liver cancer cells were selected for in vivo and in vitro experiments to study the anticancer mechanism of Sophora sophora.Results:The results showed that 39 active ingredients were screened,and 46 key targets were involved.Filtering based on degree values,the research has found that the top 20 genes are mainly enriched in related pathways such as the"cancer pathway".Molecular docking experiments showed that there was a good affinity between the key targets NF-κBp65,CASP3,STAT3 and the effective active components.The ex-perimental results of anti-hepatocellular carcinoma of Sophora flavescens showed that it promoted the apoptosis of hepatocellular carcinoma cells by inhibiting NF-κBp65 and its related factors TNF-α and IL-6.Conclusion:This study indicates that Sophora flavescens can treat HCC through multiple components,different targets,and multiple pathways,providing pharmacological data reference for elucidating the molecular mechanism of Sophora flavescens in treating HCC.
万方数据
维普
