To Investigate the Level and Clinical Significance of Biomarkers Associated with Stroke-Related Pneumonia
Objective:In this study,a prepared nanomaterial sensor was modified based on the chemical properties and material structure of the confirmed Stroke-associated pneumonia(SAP)biomarker to enable the detection of the SAP biomarker and to evaluate the differential diagnosis of SAP from non-SAP.Methods:The ischemic SAP model rats were established by photochemical method,and the hemorrhagic SAP model was established by collagenase injection method.Then the rats were placed in a fixed box,wiped the tail of the rats with alcohol,pressed 1/3 of the tail vein,injected Lipopolysaccharide after the blood was extracted from the syringe into the needle.LPS)solution(LPS dissolved in normal saline and administered at a dose of 10mg/kg)was used to establish a rat model of acute lung injury induced by endotoxin.Take a blood sample and place it in a test tube without anticoagulants;Natural agglutination at room temperature for 2-4h(anti-sun exposure),blood coagulation after the natural precipitation of the serum;Then fluorescence detection of the identified biomarkers was performed based on up-conversion nanomaterials.The Receiver Operating Characteristic Curve(ROC curve)was used to evaluate the differential diagnosis of SAP and non-SAP.Results:There were no statistical differences in Procalcitonin(PCT)and Uric acid(UA)levels between SAP and non-SAP rats,while C-reactive protein(CRP)levels in SAP group were significantly higher than those in non-SAP group.The difference was statistically significant(P＜0.05).ROC curve was used to evaluate the value of main biomarker levels in differentiating SAP and non-SAP rats.The results showed that CRP level had high clinical value in differentiating SAP and non-SAP rats(P＜0.05).Conclusion:There is a significant difference in CRP between SAP and non-SAP rats,but there is no significant difference in PCT and uric acid.CRP can be used as a reliable index to identify SAP and non-SAP rats.
Stroke-related pneumoniaBiomarkersC-reactive protein
万方数据
维普
