Objective:To explore the application value of plasma dilution regression method in eliminating the interference of fatty blood samples on coagulation results.Methods:84 normal plasma samples that underwent health examinations in our hospital from January 2021 to February 2023 were selected as the control group.Fat emulsion was prepared to simulate varying degrees of fatty blood,including 28 mild fatty blood samples,27 moderate fatty blood samples,and 29 severe fatty blood samples.Detect the coagulation function indicators of patients.During the same period,68 fat samples from outpatient and inpatient patients in our hospital were selected as the observation group(31 severe fat blood samples and 37 moderate fat blood samples).High speed centrifugation and plasma dilution regression methods were used to compare the differences in coagulation function before and after treatment with different degrees of fat blood plasma dilution regression method and high-speed centrifugation method.Results:In simulated fatty blood,the prothrombin time(PT)and activated partial thromboplastin time(APTT)values of the moderate and severe fat groups were higher than those of the control group,and the D-dimer(DD)value was lower than that of the control group,with sta-tistical differences(P＜0.05);There was no statistically significant difference in PT,DD,and APTT values between the mild fat group and the control group(P＞0.05);In the samples of moderate fatty blood,PT and APTT values were lower than before treatment after plasma dilution regression and high-speed centrifugation,and DD values were higher than before treatment,with statistical differences(P＜0.05);There was no statistically significant difference(P＞0.05)in the levels of PT,DD,and APTT after plasma dilution regression and high-speed centrifugation treatment.After plasma dilution regression and high-speed centrifugation,the PT and APTT values of severe fatty blood samples were lower than before treatment,and the DD values were higher than before treatment,with statistical differences(P＜0.05);There was no statistically significant difference(P＞0.05)in the levels of PT,DD,and APTT after plasma dilution regression and high-speed centrifugation treatment.Conclusion:Both plasma dilution regression method and high-speed centrifugation method can eliminate the interference caused by medium and heavy fat blood samples on coagulation results,but the former does not require centrifugation operation and is simpler to operate.
维普
万方数据
