A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method
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NETL
NSTL
维普
万方数据
[Objective] The study aimed to introduea a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [Method] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the spe-cifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [Result] The clear and orderly bands were observed in gel detection, and the val-ues of OD260/OD280 for DNAs extracted via modified CTAB method were between 1.77 - I. 83. The DNAs performed well in PCR amplification. [Conclu-sion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.
AnimalPlantExtraction of genomic DNAModified CTAB method
闫苗苗、魏光成、潘效红、马怀雷、李伟振
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滨州医学院烟台校区,山东烟台,264003
Animal Plant Extraction of genomic DNA Modified CTAB method
NETL
NSTL
维普
万方数据
