Cloning and Sequence Analysis of Actin Gene from Rehmannia glutinosa
[Objective] The aim of this study is to clone and analyze the actin gene from Rehmannia glutina. [Method] Degenerate primers were de-signed according to the conserved regions of actin sequences of Rehmannia glutinosa and its similar species, RT-PCR was next conducted to amplify the ac-tin gene from Rehmannia glutinosa. [Result] The amplified fragment is 724 bp and correspondingly 240 amino acids. The BLAST results indicate that the homology between the amplified fragment and other higher plants for actin gene sequences and amino acid are more than 80% and 90%, respectively, sug-gesting that the amplified fragment is the actin gene ofRehmannia ghainosa. [Conclusion] Phylogenetic analysis shows that the actin gene of Rehmannia glutinosa has an intimate genetic relationship with actin7 gene of Nicotiana tabacum.
NETL
NSTL
万方数据
维普
