Cloning and Characterization of WD40 Gene in Cotton
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A pair of PCR primers was designed based on the conserved sequences of WD40 family gene of different varieties. The normolization library was screened by PCR methods, the cDNA insert size of positive clones were analyzed by PCR method. A full-length cDNA of WD40 (GenBank accession number: EU219610) in cotton was obtained from sequencing. This gene is 1 796 bp in length, containing an open reading frame encoding 274 amino acids and a stop codon from 35th to 860th position. The bioinformatics characterization indicates that the protein is encoded by WD40 domain. pI and molecular weight of the protein encoded were predicted to be 4.24 and 29 kDa, respectively. The yielded gene was accondingly named as GDRP1. RT-PCR analysis showed that the expression of GDRP1 varied during the gland formation process. These results will be helpful for our further study on the gland formation of cotton.
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