首页|丹参药渣中提取丹参酮的研究(摘要)

丹参药渣中提取丹参酮的研究(摘要)

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[目的]水浸丹参和三七后药渣中提取丹参酮并确定丹参酮成分.[方法]采用有机溶剂法提取丹参药渣中丹参酮,7种有机溶剂分别为甲醇、正己烷、石油醚、乙酸乙酯、丙酮、乙醚、二氯甲烷、三氯甲烷.以薄层层析法检测确定最佳提取溶剂.在薄层层析板上,用毛细管吸取标准品及样品,点样于薄层层析板上,每次点样依照样品浓度确定点样次数,每次点样均须用电吹风吹干.丹参酮展开剂的配方为:石油醚:乙酸乙酯:冰乙酸=8:3:1(V:V:V),直接观察;三七皂苷展开剂为V(氯仿):V(甲醇):V(水)=14:6:1,用10%H2SO4加热显色.通过高效液相色谱法确定丹参药渣中丹参酮成分.高效液相色谱法(HPLC):高效液相色谱仪为美国Waters 2695-2996 DAD;色谱柱为Hypersil ODS2 5μm(4.6 μm×150.0 mm)from Elite;进样量,10μl;体积流量,1.0 ml/min.洗脱液A为水,B为甲醇.洗脱条件,初始时,A为40%,B为60%;5 min时,A为40%,B为60%;20 min时,A为20%,B为80%;30 min时,A为20%,B为80%.[结果]乙醚为丹参药渣中提取丹参酮的最佳溶剂.水浸丹参和三七后干药渣,先经乙醇提取得到脂溶性提取物,再以乙醚为溶剂进行索氏提取,丹参酮混合物得率为2.17%;高效液相色谱法检测,丹参酮混合物中丹参酮ⅡA、次甲丹参醌、隐丹参酮、丹参酮I含量分别为3.62%、1.02%、2.56%、2.75%.[结论]丹参药渣中丹参酮成分与药材丹参基本一致,丹参药渣可以作为一种丹参酮资源,具有开发利用的价值.
Study on Tanshinone Extracted from Salvia miltiorrhiza Bge.Dregs
[Objective]The aim was to extract tanshinone from Salvia miltiorrhiza Bge.dregs and to determine tanshinone components.[Method]Organic solvent method was adopted to extract tanshinone from S.miltiorrhiza dregs and TLC was used to determine the optimum extraction solvent.The components of tanshinone were measured with HPLC[Result]Ether was the best solvent to extract tanshinone from S.miltiorrhiza dregs.After water immersion,dry dregs of S.rniltiorrhiza and Panax notoginseng ware extracted with ethanol to obtain fat-soluble extracts.Then with ether as the solvent for Soxhlet extraction,the yield of crude tanshinone was 2.17%.The HPLC detection showed that the contents of tanshinone Ⅱ A,methylene tanshinquinone,cryptotanshinone,tanshinone Ⅰ were 3.62%,1.02%,2.56%,2.75% respectively.[Coaclusioa]The components of tanshinone in S.miltiorrhiza dregs were basically the same as tanshinone in medicine S.miltiorrhiza.S.miltiorrhiza dregs could be used as a kind of tanshinone resource,which has the value of development and utilization.

Salvia miltiorrhiza Bge.dregsTanshinoneExtraction

石岭、鱼红闪、金凤燮

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大连工业大学生物与食品工程学院,辽宁大连,116034

丹参药渣 丹参酮 提取

国家自然科学基金辽宁省教育厅创新团队项目

304700552007T006

2010

农业科学与技术(英文版)
湖南省农业科学院

农业科学与技术(英文版)

CSTPCD
影响因子：0.222
ISSN：1009-4229
年,卷(期)：2010.11(1)
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