果蝇生殖包囊固定染色后的荧光特性
Fluorescent Features of Germline Cysts of Fruit Fly ( Drosophila melanogaster) after Fixation and Staining
王冰 1王晶 1李纪同 1杨磊 1张永忠1
作者信息
- 1. 聊城大学生命科学学院,山东聊城,252059
- 折叠
摘要
[目的]探索果蝇卵巢及生殖包囊经过常规固定和染色后的荧光特性,为荧光组织学的发展提供第一手资料.[方法]以黑腹果蝇卵巢为试验材料,用Bouin氏液固定,石蜡切片后分别用HE、苏木精、伊红染色,分别在明场(白光)、绿色、蓝色和紫外激发光下观察、拍照.[结果]3种方法染色后,再经不同激发光激发,生殖细胞和体细胞可发出不同颜色的荧光,细胞中脂类、核酸及蛋白质也可分别发出各自特异性的荧光.[结论]常规染色剂可以分别赋予生殖细胞和体细胞各自不同的荧光特性,也可赋予细胞内不同物质成份各自不同的荧光特性.
Abstract
[ Objective] In order to provide firsthand information for the development of fluorescent histology, the paper studied the fluorescent features of ovarioles and germline cysts of fruit fly after common fixation and staining. [ Method] With ovaries of Drosophila melanogasteras materials, after fixation with Bouin's fluid, the ovaries were sectioned and stained with HE, haematoxylin or eosin, respectively. The specimens werk observed and photographed under fluorescent microscope in bright field, and fluorescent fields after excitation with green, blue and UV light, respectively. [ Result ] After staining by three methods, germ cells and somatic cells emitted different colors of fluorescence after excitation by different lights; lipids, nucleic acids and proteins in cells could also emit their special fluorescence. [ Condusion] Conventional dyes could give different fluorescence characteristics to germ cells and somatic cells, which can also give special fluorescence characteristics to different cellular components. Thus, the fluorescence histology will provide broad prospect for more convenient study on different cell types and cellular components.
关键词
果蝇/生殖包囊/苏木精/伊红/荧光Key words
Fruit fly/Ovary/Haematoxylin/Eosin/Fluorescence引用本文复制引用
出版年
2011
国家科技期刊平台
NSTL
万方数据