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砀山酥梨果实发育过程中内源激素含量变化动态

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[目的]明确砀山酥梨果实的生长发育与其果肉中内源激素含量的关系[方法]以砀山酥梨为试材,利用酶联免疫吸附分析法对砀山酥梨不同发育时期果肉内源激素变化动态进行了测定分析。[结果]生长促进型激素ZR、GA和IAA含量在砀山酥梨授粉后1 -15 d呈上升趋势,较高ZR、GA和IAA含量能促进砀山酥梨子房发育和正常坐果。果实中ZR含量在砀山酥梨第一个快速生长期开始时最高,之后呈下降趋势,直到果实成熟。IAA和GA的变化动态大致相似,在果实第一个快速生长期含量较高,随着果实发育逐渐减少,进入果实第二个快速生长期又迅速增加并达到最大值,与果实生长动态基本吻合。ABA含量分别在授粉后15、45、120 d出现高峰,与砀山酥梨生理落果和果实成熟时期基本一致。[结论]砀山酥梨果实的生长发育各时期持续时间及其内源激素含量也不相同,果实的生长发育与其内源激素的含量有密切关系。
Dynamic Changes of Endogenous Hormone Content in Dangshansu Pear during Fruit Development
[Objective] The aim was to clear the relationship between fruit growth and development and endogenous hormones content in the pulp of Dangshansu pear.[Method] Choosing Dangshansu pear as material,the dynamic changes of endogenous hormones in pulp during various development stages were determined by means of enzyme linked immunosorbent assay(ELISA).[Result] The results indicated that the content of endogenous hormones like ZR,GA and IAA promoting fruit growth showed increase trend within 15 d after pollination,and higher content of ZR,GA and IAA could promote ovary development and normal fruit setting of Dangshansu pear.The content of ZR in pulp had the highest value at the beginning of the first fast growth period,and then showed decrease trend until fruit became mature.The dynamic changes of IAA and GA were similar,namely their contents were higher in the first fast growth period,and decreased with fruit development,and then increased rapidly and was up to the maximum value in the second fast growth period,corresponding with the growth dynamic of fruit.The peaks of ABA content could be found on the 15th,45th and 120th day after pollination,which corresponded with the physiological fruit dropping and maturing period of Dangshansu pear.[Conclusion] The duration of various development periods and content of endogenous hormones of Dangshansu pear were different,and fruit growth and development were closely related to the content of endogenous hormones.

Dangshansu pearEndogenous hormonesFruit development

张昂、徐义流、潘海发、张金云、高正辉、束冰、郭传翔

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安徽农业大学生命科学学院,安徽合肥230036

安徽省农业科学院园艺研究所,安徽合肥230031

砀山酥梨 内源激素 果实发育

Natural Science Foundation of Anhui Province "Studyon the Metaxenia Phenomenon and its Mechanism of DangshansuPear"Anhui Agricultural Committee " Modern Agricultural Fruit Industry Technology System" (Anhui Academy of Agricultural SciencesAnhui Provincial Finance Department " Modern Agricultural Production and Development Project oDangshansu Pear Industry of Anhui

090411008[2011] No. 6[2010] No. 53

2011

农业科学与技术(英文版)
湖南省农业科学院

农业科学与技术(英文版)

影响因子:0.222
ISSN:1009-4229
年,卷(期):2011.12(7)
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