广西东兰乌鸡的遗传多样性分析
Genetic Diversity Analysis of Guangxi Donglan Black-bone Chicken
廖玉英 1莫国东 1黄英飞 1吴强 1韦凤英1
作者信息
- 1. 广西畜牧研究所/广西家畜遗传改良重点实验室,广西南宁530001
- 折叠
摘要
[目的]了解广西东兰乌鸡的遗传多样性及保种效果,为下一步的种质资源保护和育种研究提供参考依据.[方法]采集片羽东兰乌鸡和丝羽东兰乌鸡的翅静脉血样,提取总DNA,PCR扩增18个微卫星位点并对其基因片段进行扫描,利用PopGen 32生物软件分析测定东兰乌鸡群体的等位基因数(Na)、有效等位基因数(Ne)、杂合度(H)、多态信息含量(PIC),并与其他广西地方品种鸡进行聚类分析.[结果] 18个微卫星标记在广西东兰乌鸡群中均表现出较高的多态性,从所检测的80个东兰乌鸡样本中共检测出98个等位基因,每个位点的平均Na为5.44个,Ne为2.78个,PIC为0.5382,H为0.5963.其中,丝羽东兰乌鸡和片羽东兰乌鸡分别检测到93和98个等位基因,平均每个位点的Ne分别为2.62和2.76个,PIC为0.508 8和0.539 8,H为0.624 7和0.599 1.聚类分析结果显示,东兰乌鸡与广西三黄鸡、霞烟鸡、南丹瑶鸡的遗传距离(DA)均较远,与其品种的进化、地理分布和选育历史基本相符.[结论]广西东兰乌鸡保种群的遗传变异较大,具有丰富的遗传多样性,其选育潜力较高.
Abstract
[Objective] This study aimed to understand the genetic diversity and breed conservation effect,and to provide reference for future germplasm resources protection and breeding researches of Guangxi Donglan black-bone chicken.[Method] The wing venous blood of Donglan Black-bone chicken (silky feather and contour feather) was collected,and the total DNA was extracted.The 18 microsatellite loci were amplified by PCR technology,and the obtained gene fragments were scanned.The number of alleles (Na),number of effective alleles (Ne),heterozygosity (H) and polymorphism information content (PIC) were determined using PopGen 32 biological software to do cluster analysis of Guangxi indigenous chicken breeds.[Result] The 18 microsatellite loci of Donglan black-bone chicken were found to be highly polymorphic.A total of 98 alleles were detected out from 80 Donglan black-bone chicken samples.The averages of Na,Ne,PIC and H per locus were 5.44,2.78,0.538 2 and 0.596 3,respectively.A total of 93 and 98 alleles were detected out from silky-and contour-feather Donglan black-bone chickens,respectively.The averages of Ne per locus were 2.62 and 2.76,of PIC per locus were 0.508 8 and 0.539 8,of H per locus were 0.624 7 and 0.599 1,respectively.The results of cluster analysis showed that there was greater genetic distance between Donglan black-bone chicken and any of other Guangxi indigenous chicken breeds,including three yellow chicken,Xiayan chicken and Nandan Yao chicken,which was consistent with the breed evolution,geographical distribution and breeding history of Donglan black-bone chicken.[Conclusion] There are greater genetic variation and abundant genetic diversity in the conserved population of Donglan black-bone chicken,suggesting abundant genetic diversity and great breeding potential of Donglan black-bone chicken.
关键词
东兰乌鸡/微卫星标记/等位基因/遗传多样性/聚类分析Key words
Donglan black-bone chicken/Microsatellite marker/Allele/Genetic diversity/Cluster analysis引用本文复制引用
基金项目
广西自然科学基金(2013GXNSFDA019013)
广西畜牧研究所基本科研业务费专项项目(桂牧研科2014-05)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据