High-frequency Plant Regeneration from Leaf Cultures of Moringa oleifera Lam: a Multipurpose Plant
[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research.[Method] Tissue culture techniques and large scale micropropagation of M.oleifera were studied in this paper.By means of a series of experiments,we used the leaf of aseptic seedling from M.oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis.[Result] It was observed that using the fresh shelled M.o/eifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect.The seed germination rate was 85%.The leaf could produce cluster buds well using the medium with MS+2.0 mg/L 6-BA+0.05 mg/L NAA,while the best proliferation condition was under MS+6-BA 1.0 mg/L+KT 0.1 mg/L+2,4-D 2.0 mg/L+NAA 0.05 mg/L.The best rooting induction culture medium was MS+0.5 mg/L IBA,with the rooting rate as 100%.[Conclusion] This protocol might find use in mass production of trueto-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.