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辣木组织培养及高频率植株再生

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[目的]为辣木组培快繁及规模化育苗提供技术参考.[方法]以优选辣木无菌苗的真叶为材料,采用组织培养技术对其诱导、增殖、生根等问题进行研究.[结果]新鲜种子去壳后用0.1%升汞处理6 min的消毒效果最好,成功率达85%.辣木无菌苗真叶不定芽诱导及增殖最佳培养基分别为:MS+6-BA 1.0 mg/L+KT0.1 mg/L+2,4-D 2.0 mg/L+NAA 0.05 mg/L、MS+0.5 mg/L 6-BA+0.03 mg/L NAA,丛生芽明显.辣木组培苗最佳生根培养基为MS+0.5mg/L IBA,生根率可达100%,炼苗移栽后成活率达98%以上.[结论]辣木外植体的离体培养表明,不同的激素组合及其浓度对辣木外植体诱导效果差别大,各种激素经过优化配合,可以获得更高的诱导率,本研究为辣木的无菌快繁奠定应用基础,并为辣木基因工程育种等研究提供技术参考.
High-frequency Plant Regeneration from Leaf Cultures of Moringa oleifera Lam: a Multipurpose Plant
[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research.[Method] Tissue culture techniques and large scale micropropagation of M.oleifera were studied in this paper.By means of a series of experiments,we used the leaf of aseptic seedling from M.oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis.[Result] It was observed that using the fresh shelled M.o/eifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect.The seed germination rate was 85%.The leaf could produce cluster buds well using the medium with MS+2.0 mg/L 6-BA+0.05 mg/L NAA,while the best proliferation condition was under MS+6-BA 1.0 mg/L+KT 0.1 mg/L+2,4-D 2.0 mg/L+NAA 0.05 mg/L.The best rooting induction culture medium was MS+0.5 mg/L IBA,with the rooting rate as 100%.[Conclusion] This protocol might find use in mass production of trueto-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.

Moringa oleiferaTissue cultureProliferationRootingOptimization

李国葱、周启艳、李欣

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广西壮族自治区钦州市环境保护监测站,广西钦州535000

辣木 组织培养 增殖 生根 体系优化

国家自然科学基金

21165008

2016

农业科学与技术(英文版)
湖南省农业科学院

农业科学与技术(英文版)

影响因子:0.222
ISSN:1009-4229
年,卷(期):2016.17(6)
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