Coniothyrium minitans Affects Overwintering Growth and Soil Microbial Distribution of Brassica napus"Xiangzayou 787"
This study aimed to investigate the effect of Coniothyrium minitans on the growth and frost tolerance of Brassica napus L.and the microbial distribution in soil.Two treatments of blank control and application of C.minitans were designed,and the growth and frost tolerance of B.napus'Xiangzayou 787'and the microbial distribution in soil were compared between the two treatments at the seedling and flowering stages.The results showed that C.minitans improved the growth of B.napus,especially at the flowering stage,compared with the blank control.Meanwhile,it improved the frost tolerance of B.napus.The 16S rDNA amplicon sequencing results of soil samples showed that Planctomycetota,Proteobacteria,Chlorofexi,and Acidobacteriota were the dominant phyla.Compared with the blank control,the application of C.minitans decreased the relative abundance of Planctomycetota,Actinobacteriota,and Cyamobacteria and increased the relative abundance of Desulfobacterota,Acidobacteriota,and Myxococcota.Gemmataceae unclassified,Sphingomonas,Anaerolinea,Gemmata,and Planctomycetales unclassified were the dominant genera.The application of C.minitan improved the structure of microbial community in soil,thereby promoting the growth and enhancing the frost tolerance of B.napus.