噬菌核霉对油菜长势、抗冻性及土壤微生物分布的影响研究
Coniothyrium minitans Affects Overwintering Growth and Soil Microbial Distribution of Brassica napus"Xiangzayou 787"
刘朝亮 1张维 2张家清 3孙海彦 4孙玉洁5
作者信息
- 1. 湖南省农作物种子救灾储备中心,湖南 长沙,410016
- 2. 湖南隆平油料种业有限公司,湖南 长沙,410128
- 3. 湖南省种子质量检测中心,湖南 长沙,410016
- 4. 中国热带农业科学院热带生物技术研究所,海南 海口,572829
- 5. 袁隆平农业高科技股份有限公司,湖南 长沙 410125
- 折叠
摘要
为探明噬菌核霉对油菜长势、抗性及土壤微生物分布的影响,以湘杂油 787 为材料,设置空白对照和施用噬菌核霉 2 个处理,分析油菜苗期和花期的长势、抗性及土壤微生物分布的差异.结果表明:噬菌核霉处理的油菜长势较对照有明显提高,花期优势更明显,抗冻性较对照也有大幅提升;通过对土壤微生物 16S rDNA扩増子测序分析发现,门水平浮霉菌门(Planctomycetota)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)和酸性菌门(Acidobacteriota)为优势菌门;嗜菌核霉处理的浮霉菌门(Planctomycetota)、酸性菌门(Actinobacteriota)和蓝藻门(Cyanobacteria)相对丰度均低于对照,但粘菌门(Myx-ococcota)、脱硫菌门(Desulfobacterota)和酸性菌门(Acidobacteriota)的相对丰度均高于对照;属水平芽菌科未知属(Gemmataceae_unclassified)、鞘脂单胞菌属(Sphingomonas)、厌氧绳菌属(Anaerolinea)、出芽菌属(Gemmata)、浮霉菌科未知属(Planctomycetales_unclassified)5 个属为优势菌属.施用噬菌核霉可以有效改善油菜土壤微生物群落结构,从而促进其生长和抗冻性的提升.
Abstract
This study aimed to investigate the effect of Coniothyrium minitans on the growth and frost tolerance of Brassica napus L.and the microbial distribution in soil.Two treatments of blank control and application of C.minitans were designed,and the growth and frost tolerance of B.napus'Xiangzayou 787'and the microbial distribution in soil were compared between the two treatments at the seedling and flowering stages.The results showed that C.minitans improved the growth of B.napus,especially at the flowering stage,compared with the blank control.Meanwhile,it improved the frost tolerance of B.napus.The 16S rDNA amplicon sequencing results of soil samples showed that Planctomycetota,Proteobacteria,Chlorofexi,and Acidobacteriota were the dominant phyla.Compared with the blank control,the application of C.minitans decreased the relative abundance of Planctomycetota,Actinobacteriota,and Cyamobacteria and increased the relative abundance of Desulfobacterota,Acidobacteriota,and Myxococcota.Gemmataceae unclassified,Sphingomonas,Anaerolinea,Gemmata,and Planctomycetales unclassified were the dominant genera.The application of C.minitan improved the structure of microbial community in soil,thereby promoting the growth and enhancing the frost tolerance of B.napus.
关键词
湘杂油787/噬菌核霉/16S/rDNA测序/微生物覆盖率Key words
Xiangzayou 787/Coniothyrium minitans/16S rDNA sequencing/Microbial coverage引用本文复制引用
基金项目
科技创新 2030-"农业生物育种"重大项目(2023ZD0404205)
出版年
2024
NETL
NSTL
万方数据