Effect of Different Disinfection Methods on Calluses of Amorphophallus albus P.Y.Liu&J.F.Chen
This study aimed to examine the effect of different disinfection methods on the callus of Amorphophallus albus in the tissue culture and rapid propagation system.The calluses induced from buds of A.albus were disinfected with benzalkonium bromide,mercury bichloride,sodium hypochlorite,and ethanol,respectively,for different time periods.The growth of calluses was compared between different treatments and observed in detail by dissection.The results showed that the disinfection with 0.1%mercury bichloride for 5 min and 75%ethanol for 5 s had mild effect on the callus,with the lowest browning rates of 16.32%and 15.46%and the lowest vitrification rates of 3.24%and 9.72%,respectively,after 20 d.The disinfection with benzalkonium bromide and sodium hypochlorite had strong effect on the callus cells,with the lowest browning rate of 50.69%and 25.12%and the lowest vitrification rates of 28.47%and 28.43%,respectively.The cytological observation result showed that the calluses disinfected with ethanol and mercury bichloride showcased compact structures,while those disinfected with sodium hypochlorite and benzalkonium bromide exhibited loose structures.To sum up,it is recommended that 75%ethanol or 0.1%mercury bichloride can be used for disinfection in the tissue culture and rapid propagation of A.albus,while the use of benzalkonium bromide and sodium hypochlorite should be avoided as much as possible.
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