Cloning of BjNAC102 Promoter and Construction of Expression Vector in Brassica juncea
Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3 000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea'Sichuan Yellow Seed'by using the homologous cloning method.The expression vector of the GUS gene driven by the BjNAC102 promoter was constructed by seamless cloning technology.The results showed that the sequence of the promoter of the BjNAC102 gene contained many cis-acting elements involved in light responsiveness,gibberellin-responsive element,and auxin-responsive element.It was speculated that BjNAC102 played an important role in the abiotic stress response in Brassica juncea.The expression vector of the promoter of the BjNAC102 gene was constructed,which layed a foundation for further studies of the expression pattern of the BjNAC102 gene in Brassica juncea.
Brassica junceaPromoterBjNAC102Plant expression vector construction
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