山银花中酚类物质提取工艺优化及其成分初鉴
Extraction Condition Optimization and Identification of Phenols from Lonicerae flos
高炜 1李浪 2余莲芳 3丁慧娟 3丁胜华1
作者信息
- 1. 湖南省农业科学院农产品加工研究所,湖南 长沙 410125
- 2. 岳阳市农业科学研究院,农业农村部植物新品种测试岳阳分中心,湖南 岳阳 414000
- 3. 岳阳市检验检测中心,湖南 岳阳 414000
- 折叠
摘要
以自然干燥山银花为原料,采用超声波辅助乙醇溶剂提取法,通过单因素试验,结合响应面对山银花多酚的提取工艺进行优化,并用超高效液相色谱串联质谱(UPLC-MS/MS)基于植物广泛靶向数据库对其多酚物质进行定性定量初鉴.结果表明:山银花总酚提取量的最佳工艺条件为:料(g)液(mL)比 10∶1,乙醇浓度 57%,超声功率 70 W,提取温度 60℃,提取时间 11 min,此条件下山银花总酚提取量为 71.08 mg/g;山银花酚酸以绿原酸同分异构体为主,黄酮以川陈皮素、木犀草苷和香叶木苷为主.
Abstract
Polyphenols were obtained from the natural dried Lonicerae flos by ultrasound-assisted extraction with ethanol as the solvent.Single factor experiment and response surface methodology were employed to optimize the extraction conditions.Ultra-performance liquid chromatrography(UPLC)-tandem mass spectrometry(MS/MS)was employed to identify polyphenols based on the plant widely targeted metabolomics database in a qualitative and quantitative manner.The results showed that the optimal extraction conditions for total phenols from Lonicerae flos were ultrasound-assisted extraction with a solid-to-liquid ratio of 10:1 g/mL and 57％ethanol at 70 W and 60 ℃ for 11 min.The yield of total phenols extracted under the optimal conditions reached 71.08 mg/g.The phenols in Lonicerae flos were mainly chlorogenic acid isomers,and the flavonoids were mainly nobiletin,galuteolin,and homoarbutin.
关键词
山银花/总酚/超高效液相色谱串联质谱/响应面法/酚类物质Key words
Lonicera flos/Total phenols/Ultra-performance liquid chromatrography(UPLC)-tandem mass spectrometry(MS/MS)/Response surface methodology/Phenols引用本文复制引用
出版年
2024
NETL
NSTL
万方数据