Inhibition Effects of Ethanol Extracts from Eupatorium adenophorum Spreng on Intestinal Bacteria from 5th Instar Larvae of Dendrolimus kikuchii
The Oxford cup method was used to measure the inhibition effects of ethanol extracts from different parts of Eupatorium adenophorum Spreng on intestinal bacteria from 5th instar larvae of Dendrolimu kikuchii. The results showed that except stems the ethanol extracts from leaves and roots of E. Adenophorum exhibited inhibition effects on 10 strains of intestinal bacteria and the effect order of different parts was leaves>roots>stems. Under the same concentration of leaf extracts, the order of inhibition effects on intestinal bacteria was Yokenella regensburgei^> Citrobacter freundii^> Klebsiella oxytoca^>Klebsiella peneumoniae~>Bacillus thuringiensis. The minimum inhibition concentration (MIC) was 0. 125 g/mL on Yokenella regensburgei,Klebsiella oxytoca and Citrobacter freundii ,0. 25 and 0. 5 g/mL on other 2 bacterial species. The root ex-tracts had no inhibition effects on Yokenella regensburgei and Klebsiella peneumoniae. Under the same concentration,the order of inhibition effects on other 3 bacterial species was consistent with that of leaf extracts,and the MIC of root extracts was 0. 25 g/mL. The ethanol extracts from E. Adenophorum had good inhibition effects on intestinal bacteria and could be used to develop new plant source bio-pesticide.