Physiological Characters Analysis and Gene Fine Mapping of Green-revertible Albino Mutation Line Bai 784
In order to study the physiological characters and relative genetic mechanism of green-revertible albino mutation line Bai 784,the phenotype and physiological characters were studied under different temperature.The results showed that the albino phenotype was controlled by temperature.Under the temperature of 20 ℃ ,22 ℃ ,24 ℃ , pigment synthesis of the mutant seedling was hindered, and the mutant seedling showed albino phenotype.Compared with the control,the content of chlorophyll a,b significantly decreased by 95.18％ ,86.86％ respectively under 20 ℃for 10 d;the content of chlorophyll a,b and carotenoid significantly decreased by 96.19％ ,93.25％ ,92.76％ respectively under 22 ℃ for 14 d;the content of chlorophyll a,b and carotenoid decreased by 86.17％ ,91.67％ ,95.30％ respectively under 24 ℃ for 12 d;the mutation line turned green,and the chlorophyll a,b and carotenoid content decreased by 27.63％ ,25.70％ ,27.17％ respectively under 26 ℃ for 12 d;the mutant leaf color was nearly the same as the control under 28 ℃ for 12 d,and there was no difference in the chlorophyll a,b and carotenoid content between mutant and control,indicating that 24-26 ℃ was the sensitive temperature for albino of leaf.The gene fine mapping was done with F2 mapping population of Bai 784 × Zhengzhouzaojing, and the results showed that the albino phenotype was controlled by a single nuclear recessive gene (Abt6) ,which was mapped on chromosome 6 between SSR markers RM5998 and RM6071 with a genetic distance of 2.4 cM and 9.7 cM respectively.Screening F2 and F3 progenies for lines whose marker genotype was heterozygous in target interval,with which and some new InDel markers the target gene was delimited to a 278 kb interval between Y15420 and Y15648.
国家科技期刊平台
NSTL
万方数据
维普
