Cloning and Expressional Characterization of Wet Damage Related Gene BnLDH-1 from Brassica napus L
The BnLDH-1 gene cDNA and genomic sequences were cloned by the RACE from Zhongshuang No. 9. The genomic sequence of BnLDH-1 was 1 432 bp and contained one intron. The 1 319 bp BnLDH-1 cDNA had a 1 053 bp ORF which encoded a polypeptide of 350 amino acids. The predicted results in-dicated that the BnLDH-1 protein contained conserved LDH-1 domain. qRT-PCR results indicated that BnLDH-1 transcription levels were induced by waterlogging, and the BnLDH-1 transcription level had sig-nificant difference among the Brassica napus L. with different waterlogging tolerance after waterlogging treatment. Correlation analysis indicated that BnLDH-1 transcription levels were significantly negatively correlated with waterlogging tolerance indices of 12 Brassica napus L. at 48 h after waterlogging treat-ment, and the correlation coefficient was 0. 73.
国家科技期刊平台
NSTL
万方数据
维普
