Cloning and Expression Analysis of Transcriptional Factor Gene TabHLH041 from Wheat
In order to lay a foundation for the study of the function of bHLH transcription factors in the development of wheat,a bHLH transcription factor TabHLH041 gene was cloned by RT-PCR method from wheat variety Liaochun No.10,and its expression pattern was analyzed.The results showed that the open reading frame of TabHLH041 was 1 179 bp in length that encoded 392 amino acids.The TabHLH041 protein contained a bHLH domain in its C terminal.The analysis of the sequence of TabHLH041 from genome DNA showed that TabHLH041 gene contained four exons and three introns.Phylogenetic analysis showed that the sequence of TabHLH041 was closest to Hordeum vulgare bHLH protein.Real time PCR analysis revealed that the expression of TabHLH041 displayed the day-night rhythm,and its expression level decreased in the light while increased in the dark.The expression of TabHLH041 was induced in the early time,and then was inhibited under the treatment of low temperature,the inhibition was very significant when treated for 24 h.The expression of TabHLH041 was inhibited continuously under the treatment of high salt,ABA and PEG.In summary,the results suggest that TabHLH041 may be involved in the signal transduction pathway of photoperiod and adversity stress.
国家科技期刊平台
NSTL
万方数据
维普
