Cloning and Expression Analysis of CaCBF1A Gene from Capsicum annuum L.under Abiotic Stress
To reveal the roles of CaCBF1A gene played in pepper,the CaCBF1A gene was cloned and analyzed.According to the CBF gene sequence from Capsicum annuum genome,the primer was designed.Yujiao 101 was taken as material and CaCBF1A was acquired from the genome of pepper by PCR.The bioinformatics analysis results indicated that the gene possessed complete ORF with length of 471 bp,encoding 156 amino acids.Protein encoded by CaCBF1A gene in pepper contained conservative AP2 DNA binding domain.Besides,the subcellular localization and membrane structure were analyzedJt was predicted that protein encoded by CaCBF1A was located in chloroplasts,and contained transmembrane structure.Furthermore,the results by fluorescence quantitative PCR showed that cold,high temperature and salt stress could induce the expression of CaCBF1A gene,the expression level of which was decreased rapidly after peak point.These could provide some indication that CaCBF1A was rapid response gene,which played important roles in the resistance mechanisms of pepper.
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