Mitigative Effects of Exogenous NO on Cotton Seedlings under Severe Cd Stress
To investigate the relieving effects of exogenous nitric oxide (NO) on cotton seedlings under severe Cd stress,Nongdamian No.601 was used as test material to study the effects of different concentrations (50,100,200 μmol/L) of sodium nitroprusside (SNP,the donor of NO) on cotton seedlings under severe Cd stress(75 μmol/L)using hydroponics method.The results showed that under severe Cd stress,the plant height and leaf area of cotton seedling were significantly decreased by 40.9% and 31.9% compared with CK(without CdCl2 and SNP);dry matter accumulation and tissue water content were only 34.3% and 33.2% of CK;photosynthetic rates declined significantly.Under severe Cd stress,compared with T0 treatment (75 μmol/L CdCl2),for the treatments with 50 and 100 μmol/L NO,the plant height and leaves area increased slightly(P > 0.05),chlorophyll contents increased by 4.5% and 15.5% respectively (P > 0.05),photosynthetic rate increased by 36.6% and 27.9% (P > 0.05),and other indexes did not change significantly;however,for the treatments with 200 μmol/L NO,the dry matter accumulation,root/shoot ratio,tissue water content,and chlorophyll content decreased.Therefore,the relieving effects of exogenous NO on the growth of cotton seedlings under severe Cd stress were not significant.