Expression and Functional Analysis of Maize Stress Response Gene ZmTPR1
On the basis of previous transcriptome sequencing of maize under drought-rehydration treatment,ZmTPR1(tetratricopeptide repeat 1)was identified in response to drought stress.Bioinformatics analysis was conducted,and expression pattern was analyzed in different tissues and under different stresses.The homologous gene AtTPR1 in Arabidopsis thaliana was knocked out by CRISPR-Cas9 technology,the phenotype,physiological and biochemical indexes of homozygous mutant plants were analyzed under drought stress,and the function of this gene was preliminarily explored,which would provide genetic resources for breeding drought-resistant maize varieties.The results showed that ZmTPR1 gene was located on chromosome 3 of maize,encoding 421 amino acids,containing a conserved coiled coil domain,and might be involved in the response of maize to plant hormones,drought and so on.ZmTPR1 gene expressed in all tissues of maize,with the highest expression level in young stems.Drought,high temperature,salt and nitrogen deficiency stresses could induce the expression of ZmTPR1 gene,and the expression level of ZmTPR1 gene was most up-regulated after drought stress.The expression level of ZmTPR1 gene in drought-resistant maize inbred line Zheng 36 was significantly higher than that in drought-sensitive maize inbred line B73 after drought stress.Drought resistance of Arabidopsis thaliana decreased after AtTPR1 gene was knocked out,the growth of Attpr1 mutant was severely inhibited under drought stress,and the leaves were wilted or even dried out.At the same time,the relative water content,chlorophyll content,net photosynthetic rate,activities of peroxidase and superoxide dismutase significantly decreased,and the content of malondialdehyde significantly increased.In summary,ZmTPR1 gene is involved in the response of maize to various abiotic stresses,and plays an important role in drought stress.
万方数据
维普
