Comparison of Leaf Anatomical Atructure and Stress Resistance Analysis of Wild Tea Plants in Panzhou City and Sandu County,Guizhou Province
To better develop and utilize wild tea germplasms in Panzhou City and Sandu County of Guizhou Province,a total of 24 wild tea plants distributed in the two areas were used as materials,and 23 leaf traits derived from their leaf morphology and anatomical structure characteristics were comparatively analyzed.Based on these traits and combined with the membership function method,the resistance of wild tea plants was analyzed.The results showed that there were obvious differences in leaf appearance between wild tea plants in Panzhou and Sandu.Among them,the leaf length(LL),leaf width(LW)and leaf area(LA)of wild tea plants in Panzhou were significantly larger than that of Sandu,but the leaf length-to-width ratio(LR)was the opposite.In addition,there were significant or extremely significant differences in 16 leaf anatomical structural traits such as leaf thickness(LT),thickness of palisade tissue(TP),cell amount(CA),main vein thickness(MVT)and density of calcium oxalate crystal(DCO)between wild tea plants in Panzhou and Sandu.Among them,the LT,TP and MVT of Panzhou wild tea plants were thicker,palisade tissue cells were more densely arranged,calcium oxalate crystals were more dense,and the thickness of upper(lower)epidermis cells was thinner.The pairwise correlation analysis of the above 23 leaf traits showed that 43 pairs of them were highly significantly positively correlated,23 pairs were significantly positively correlated,17 pairs were highly significantly negatively correlated,and 12 pairs were significantly negatively correlated.Cluster analysis showed that the 24 wild tea plants could be divided into 4 categories,with the MVT and short diameter of vascular bundle(SDV)being the main basis for the classification.The results of stress resistance evaluation showed that Panzhou wild tea plants were slightly better than Sandu wild tea plants in drought and cold resistance.According to the average membership function value,SD-20-2,SD-20-3,PZ-05 and PZ-09 were selected as high resistance wild tea germplasms for breeding resistant tea cultivars.