To elucidate the structure and sequence characteristics of chloroplast genome from Paederia scandens,the chloroplast genome sequencing was performed using high-throughput sequencing technology,and then acquired sequence was assembled,annotated and analyzed via bioinformatics procedures.The results demonstrated that the chloroplast genome of P.scandens was a circular quadripartite molecule with a length of 153456 bp and encoded 133 genes.Altogether 54 simple sequence repeats were detected in the plastome and the A/T mononucleotide was main type.A total of 26983 codons were searched out and those with end of A/T were frequently used.Sequence alignment demonstrated more divergence in non-coding region among five Rubiaceae species including P.scandens.Phylogenetic tree revealed close relationship of various chloroplast genome sequences of P.scandens and clearly displayed the evolutionary position of P.scandens in Rubiaceae.
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