Effect of Biochar on Rhizosphere Soil Fungal Community Structure of Flue-cured Tobacco Varieties with Different Resistance to Black Shank Disease
To explore the effect of biochar on the fungal community structure in rhizosphere soil of flue-cured tobacco varieties with different resistance to black shank disease,and to provide theoretical basis for regulating tobacco microenvironment and comprehensively controlling black shank disease,pot experiments were conducted with different flue-cured tobacco varieties Jingyehuang(highly susceptible variety),K326(moderately resistant variety)and NC82(highly resistant variety)as materials,and no tobacco cultivation was used as control(CK).Two levels of biochar and no biochar were set up,with a total of eight treatments,and the effect of biochar on fungal community structure in rhizosphere soil of flue-cured tobacco varieties with different resistance to black shank was explored by high-throughput sequencing technique.The results showed that,after applying biochar,the incidence and disease index of black shank disease of flue-cured tobacco varieties with different resistance to black shank disease were significantly reduced,and the control effects for highly susceptible variety Jingyehuang,moderately resistant variety K326 and highly resistant variety NC82 were 56.52%,31.00%and 27.00%respectively.Under the condition of no biochar application,the fungal diversity of rhizosphere soil of high-resistance variety NC82 and high-sensitivity variety Jingyehuang was relatively higher,while that of middle-resistance variety K326 was relatively lower.After biochar application,the fungal diversity of middle-resistance variety K326 increased significantly,while the fungal diversity of high-sensitivity variety Jingyehuang and high-resistance variety NC82 decreased in different degrees.LefSe analysis showed that the key microbial groups in rhizosphere soil of different resistant flue-cured tobacco varieties were significantly different without biochar application,and the application of biochar changed the key microbial groups in rhizosphere soil,with obvious differences among varieties.PCoA analysis showed that under the condition of no biochar application,compared with the soil without tobacco planting,the sample points of three flue-cured tobacco varieties were obviously separate,and the application of biochar changed the fungal community structure in rhizosphere soil of three flue-cured tobacco varieties.To sum up,applying biochar can significantly reduce the occurrence of tobacco black shank disease.The rhizosphere soil fungal community structure of flue-cured tobacco varieties with different resistance to black shank disease has different responses to biochar input.In production,variety factors should be considered when using biochar to improve soil and tobacco microecological environment.
万方数据
维普
