Development and Preliminary Application of Colloidal Gold Immunochromatography Test Strips for Egg Drop Syndrome Virus Antibodies
In order to establish a method for rapid detection of egg drop syndrome virus(EDSV)antibody,an immunochromatographic test strip for rapid detection of EDSV antibody was prepared based on the EDSV Fiber protein,which could provide infection warning and immune evaluation for pathogenic microorganisms in poultry industry.Colloidal gold-labeled EDSV Fiber protein was used as a probe,the goat anti-chicken IgG and anti-Fiber polyclonal antibody were blotted onto the nitrocellulose membrane as quality control lines and detection lines respectively to test EDSV antibody.The results showed that the EDSV immunochromatographic test strip was highly specific,had no cross-reactivity with other viral antibodies,it only reacted with EDSV antibodies and produced a visible detection line on the test strip;and the test strips were highly stable and can be stored for at least 6 months at room temperature.The clinical application effect of the strips was tested by detecting EDSV antibodies in 576 serum samples collected from chickens farms in Henan,Anhui and Shandong,and compared with the hemagglutination inhibition test(HI)in parallel,and the Kappa value of the two was 0.878,which was in good agreement.In summary,the immunochromatographic test strips developed in this study are highly specific,sensitive,stable and easy to operate,and have practical application value for the clinical detection of EDSV antibodies.
Egg drop syndrome virusFiber proteinImmunochromatographic stripHemagglutination inhibition test(HI)
万方数据
维普
