In order to improve the continuous sterilization processing of Lentinula edodes culture materials by using twin-screw extruder,and develop a new sterilization technology of culture materials,with the sterilization rate as index,the sterilization process was optimized by Plackett-Burman test,the steepest ascent experiment and Box-Behnken response surface test,and then the cultivation comparison experiment was carried out.The results of process optimization showed that the optimum technical conditions of twin-screw extrusion sterilization were as follows:initial moisture content of raw materials 48.6％,feeding motor frequency 27 Hz,zone Ⅲ temperature 162℃,sawdust wetting time 3 h,zone Ⅰ temperature 90℃,zone Ⅱ temperature 140℃,zone Ⅳ temperature 130℃,motor frequency of screw 21 Hz.Under this technology,the sterilization rate of Lentinula edodes culture materials by twin-screw extrusion reached 99.92％.The results of cultivation experiment showed that there was no significant difference in the weight of mushroom cultivation bag,the full bag days of mushroom mycelium,and the color conversion days between twin-screw extrusion sterilization and traditional steam sterilization.The cultivation bag of twin-screw extrusion sterilization could produce mushrooms and had good mushroom type,the single mushroom weight,mushroom cap diameter and mushroom stalk length increased significantly,while the bad bag rate of mushroom decreased significantly.Therefore,the twin-screw extrusion sterilization was applicable to the sterilization processing of mushroom culture materials.
维普
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