Isolation,Identification of Golubevia albescens 17wy1 and Exploration of Its Control Effect on Wheat Powdery Mildew
An isolate 17wy1 from abnormal conidium of Blumeria graminis f.sp.tritici(Bgt)was identified by 26S rDNA sequence analysis for classification,the inhibition effect of its fermentation broth on Bgt conidium germination and the control effect of its fermentation broth on indoor pot seedling and field wheat powdery mildew were detected,and the expression pattern of wheat pathogenesis-related genes such as PR1,PR2,PR5 and TaPAL was analyzed by RT-qPCR in order to explain antagonistic mechanism,so as to provide a basis for 17wy1 biological control on wheat powdery mildew.The results showed that isolate 17wy1 was Golubevia albescens,its fermentation broth(5×107 spores per milliliter)had 85.1%inhibition rate on Bgt conidium germination,and 58.5%control effect on wheat powdery mildew in indoor seedlings and 69.8%in field wheat,respectively.Two pathogenesis-related protein genes PR1 and PR5 were induced and the expression was significantly up-regulated in symptomatic indoor wheat seedlings after spraying 17wy1 fermentation broth.In conclusion,the yeast 17wy1 can inhibit the conidium germination of Bgt,has a good control effect on wheat powdery mildew both indoors and in the field,and may be a potential biocontrol agent against wheat powdery mildew.
Wheat powdery mildewGolubevia albescensClassification and identificationInduced resistanceControl effect
万方数据
维普
