暹罗芽孢杆菌Y-54对番茄叶霉病的生防作用
Biocontrol Effect of Bacillus siamensis Y-54 against Tomato Leaf Mold
曾婉宁 1王彦譞 1王繁珍 2陈招荣 1刘慧芹 3姜永成 4艾则麦提·图尔洪 5李宝通3
作者信息
- 1. 天津农学院 园艺园林学院,天津 300384
- 2. 天津农学院 计算机与信息工程学院,天津 300384
- 3. 天津农学院 园艺园林学院,天津 300384;和田职业技术学院,新疆 和田 848000
- 4. 天津农学院 工程技术学院,天津 300384
- 5. 和田职业技术学院,新疆 和田 848000
- 折叠
摘要
为明确暹罗芽孢杆菌Y-54对番茄叶霉病的生防作用,给番茄叶霉病的生物防治提供科学依据,采用对峙及显微观察方法测定菌株Y-54对导致番茄叶霉病的黄褐孢霉菌的抑制作用,以灌根法研究拮抗菌株Y-54在番茄中的定殖变化,采用浸种法和灌根法研究菌株Y-54对番茄种子萌发和幼苗生长的促生作用,并通过盆栽试验测定了防治效果.结果表明,菌株Y-54及其发酵液和无菌滤液均对番茄叶霉病菌有较强的抑制作用,抑制率分别达到78.42%、86.42%和91.26%;无菌滤液可使番茄叶霉病菌菌丝变形弯曲、萎缩、膨胀、断裂等;菌株Y-54在接种30d内均可在番茄根茎叶部位定殖,15～18 d时达到定殖高峰,其中在根部的定殖量始终高于其他部位.菌株Y-54可以有效地促进种子的萌发,其发酵原液(0×)可使种子发芽率达到最高,为93.33%;并且能有效促进幼苗根长、株高、鲜质量、叶绿素含量等各项指标的增长,发酵原液(0×)和10×、20×发酵液的促生作用显著高于无菌水(CK).菌株Y-54发酵原液(0×)对番茄叶霉病的防效在7d和14d时分别达68.04%和71.07%,接近于苯醚甲环唑处理,显著高于喷施多菌灵处理.综上,菌株Y-54对番茄叶霉病具有较好的防效,是一株值得深入开发利用的生防菌.
Abstract
In order to clarify the biocontrol effect of Bacillus siamensis Y-54 against tomato leaf mold and provide a scientific basis for the biological control of tomato leaf mold,the inhibitory effect of strain Y-54 on Cladosporium fulvum was measured by confrontation and microscopic observation,and the colonization of antagonistic strain Y-54 in tomato was studied by irrigation method.The growth promoting effect of the strain Y-54 on seed germination and seedling growth of tomato was studied by soaking seed method and filling root method.The control effect was measured by pot experiment.The result showed that strain Y-54 and its fermentation broth and sterile filtrate all had strong inhibitory effects on C.fulvum,with inhibition rates reaching 78.42%,86.42%and 91.26%,respectively.The sterile filtrate could make mycelium of C.fulvum deform,bend,shrink,expand and break.The strain Y-54 could colonize in the roots,stems and leaves of tomato within 30 days.The colonization of the strain Y-54 reached the peak at 15-18 d.The colonization in the root was always higher than that in other parts.Strain Y-54 could effectively promote seed germination.The germination rate of fermentation broth(0×)treatment was the highest,reaching 93.33%.Moreover,it could effectively promote the increase of root length,plant height,fresh weight,chlorophyll and other indexes of seedlings.The growth promoting effect of 0×,10×and 20×fermentation broth was significantly higher than that of the control with sterile water.The control effects of strain Y-54 fermentation broth on tomato leaf mold within 7 days and 14 days were 68.04%and 71.07%,respectively,close to that of phenoxymethyclozole treatment,and significantly higher than that of carbendazim treatment.In summary,the strain Y-54 had good control effect on tomato leaf mold,and it was a biocontrol bacterium worth further development and utilization.
关键词
番茄叶霉病/暹罗芽孢杆菌/定殖/促生作用/防治效果Key words
Tomato leaf mold/Bacillus siamensis/Colonization/Growth-promoting effect/Control effect引用本文复制引用
基金项目
河北省重点研发计划(22347402D)
新疆维吾尔自治区引进项目(第二次)(2022008)
天津市农业科技成果转化与推广项目(202101080)
山东省重点研发计划(2023TZXD034)
新疆维吾尔自治区科技成果转化示范专项(2023)()
和田地区本级技术研究与开发经费项目(20220219)
出版年
2024
国家科技期刊平台
NSTL
万方数据