Characteristics of Lignocellulose Variation in Lentinus edodes Culture Material during Different Growth Periods
In order to explore the characteristics of lignocellulose variation in Lentinus edodes culture material during different growth periods and promote its efficient degradation and utilization,with Lentinus edodes culture material as the material,the lignocellulose content,crystallinity of cellulose,lignocellulose functional groups and fiber morphology of Lentinus edodes culture material at each growth period were analyzed by Van Soest method,X-ray diffraction method,Fourier transform infrared spectroscopy(FTIR)and optical microscopic imaging.Results showed that with the growth of Lentinus edodes,the content of lignocellulose components were decreased significantly,cellulose degraded more in reproductive growth stage,and the degradation rate of hemicellulose,lignin and holocellulose accelerated after entering the color conversion period.The contents of cellulose,hemicellulose,lignin and holocellulose in the second mushroom period were reduced by 34.73%,61.58%,57.15%and 42.33%respectively compared with that in raw materials.The Lentinus edodes culture material at each growth period had typical cellulose X-ray diffraction characteristics,the crystalline region of cellulose was destroyed,and the crystallinity of typical diffraction peaks continued to decrease.FTIR showed that the main absorption peaks position of the lignocellulose infrared spectral characteristic functional groups did not change,but the intensity weakened,which was consistent with the degradation and utilization of lignocellulose components.The ratios of I1723/I1510,I1383/I1510 and I1157/I1510 showed a decreasing trend,while I897/I1510 showed an increasing trend in the color conversion and first mushroom periods,indicating that the degradation degree of lignocellulose components was different,and lignin had a faster degradation rate than cellulose in this growth period.The fiber length and width generally showed a decreasing trend,and there were significant differences between the second mushroom and other growth periods.Observed by the microscope,the cellulose wall and inner lumen in the second mushroom period were destroyed,which was obviously different from the raw material.Therefore,Lentinus edodes has a strong continuous degradation effect on the culture lignocellulose,could significantly reduce the lignocellulose content,destroy the crystalline region of cellulose,and change the infrared spectrum absorption peak and fiber morphology.
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