Investigation on Anti-MDV Active Components and Mechanism of Artemisia argyi Lévl.et Van.Based on Network Pharmacology
The purpose of this study was to explore the active components,targets and possible mechanisms of Artemisia argyi Lévl.et Van.against Marek's disease virus(MDV)by using network pharmacological methods.The TCMSP database was used to search the chemical components and action targets of Artemisia argyi Lévl.et Van.,and the MDV related host gene targets were collected through relevant literature to establish a target database;by using STRING database and Cytoscape 3.8.0 software,target protein interaction network and active ingredient-key target network were constructed to screen core targets and main active ingredients;the DAVID database was used to analyze GO function and KEGG pathway enrichment of key targets;finally,molecular docking technology was used to verify the binding ability of the main active ingredients to key targets.The results showed that eight active components were screened from Artemisia argyi Lévl.et Van.,and 108 gene targets interacted with MDV host genes.The main targets of Artemisia argyi Lévl.et Van.with anti-MDV action were JUN,CCND1,CDK1,IL-6,etc;GO analysis involved cell activity,inflammatory reaction and other processes,and KEGG enrichment analysis involved C-type lectin receptor signal pathway,MAPK signal pathway,cell aging,p53 signal pathway,apoptosis,Toll like receptor signal pathway,etc.The molecular docking results showed that the binding ability of eupatilin to the target protein CCND1 was the strongest.The results indicate that the main active component of Artemisia argyi Lévl.et Van.,eupatilin,may interfere with cell cycle and affect cell activity by acting on CCND1,thereby exerting anti-MDV effects.
国家科技期刊平台
NSTL
万方数据
