Effects of PAW and Its Combined Action with LTP on Seed Germination and Seedling Growth of Wheat
Taking Jimai 22 and Bainong 307 as materials,the effects of plasma-activated water(PAW,with preparation time of 5.0,7.5 and 10 min,named as PAW-5,PAW-7.5 and PAW-10,respectively)and its combined action with low-temperature plasma(LTP,named as LTP+W-5,LTP+W-7.5,LTP+ W-10,respectively)on wheat seed germination and seedling growth were studied,so as to provide a theoretical basis for the application of PAW and LTP in wheat seed vigor enhancement.The results showed that the germination rate and germination index of two wheat varieties reached the best at 9 s after LTP treatment,so LTP treatment time of 9 s was chosen for the study of the combined effect of PAW and LTP.The PAW treatment and the combined treatment of PAW and LTP increased the germination rate of wheat seeds,plant height,fresh and dry weight of seedlings,and significantly increased seed vigor index,and LTP+W-7.5 treatment was generally the best.The contact angle between seeds without LTP treatment and distilled water was the largest,indicating that the wettability was the weakest.In contrast,the contact angle between seeds without LTP treatment and PAW decreased,while the contact angle between seeds with LTP treatment and distilled water/PAW significantly decreased.Compared with treatment treated with distilled water,the content of reactive oxygen and nitrogen species(RONS)in wheat seedlings treated with PAW and PAW+LTP generally significantly increased,while the RONS content in wheat seedlings treated only with LTP was not significantly changed.In conclusion,PAW and PAW+LTP can effectively promote wheat seed germination and seedling growth,and LTP+W-7.5 treatment is the best.
WheatSeed germinationSeedling growthLow-temperature plasmaPlasma-activated waterVigor indexWetting propertyReactive oxygen and nitrogen species
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