Molecular Identification and Biological Characterization of Ralstonia solanacearum Infecting Peanuts in Henan Province
To identify the taxonomic properties of the pathogen of peanut bacterial wilt disease in Henan Province,30 strains collected from different regions in Henan Province were comparatively characterized in terms of 16S rDNA sequences,carbohydrate utilization,pathogenicity,evolutionary type,and sequence variation.The results showed that,based on the 16S rDNA sequencing results of these 30 strains,the pathogen causing peanut wilt disease was R.solanacearum;all the strains could infect eggplant,chili pepper,potato,tobacco,and tomato,but did not infect ginger and belonged to physiological race 1;based on the analysis of the ability to utilize three disaccharides and three hexanols,four strains were classified as biovarⅡ,10 strains as biovarⅢ,and 16 strains as biovar Ⅴ;all the strains were able to produce 144 bp specific band for evolutionary typeⅠand 280 bp specific band for R.solanacearum through multiplex PCR amplification,which indicated that all of the strains belonged to the evolutionary type Ⅰ,i.e.,the Asian group 13 of R.solanacearum;based on the phylogenetic analysis of the egl gene,all the strains from Henan were clustered together with Gx525,Ah-XnJn-12-6,and HA2-1,belonging to sequence variant 14.
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