Comprehensive Evaluation of Photosynthetic Performance of Foxtail Millet Varieties and Screening of High Photosynthetic Efficiency Varieties
A field experiment was conducted to determine the photosynthetic indexes[intercellular CO2concentration(X1),transpiration rate(X2),stomatal conductance(X3),net photosynthetic rate(X4),instantaneous water use efficiency(X5)]and leaf morphological indexes[leaf area(X6),dry leaf weight(X7),specific leaf weight(X8)]of 37 foxtail millet varieties,the fuzzy membership function,principal component analysis,correlation analysis and cluster analysis were used to comprehensively evaluate the photosynthetic performance of foxtail millet varieties,the varieties with high photosynthetic efficiency were screened,and the regression equation of photosynthetic performance prediction was established by stepwise regression analysis,so as to provide a reference for the screening and utilization of high photosynthetic efficiency foxtail millet germplasm.The results showed that the photosynthetic indexes and leaf morphological indexes were significantly different among 37 foxtail millet varieties.The CV ranged from 3.41%to 33.94%.The CV of stomatal conductance was the largest(33.94%),and the CV of specific leaf weight was the smallest(3.41%).The results of correlation analysis showed that intercellular CO2 concentration was significantly negatively correlated with transpiration rate and net photosynthetic rate;Transpiration rate was significantly positively correlated with net photosynthetic rate;Instantaneous water use efficiency was significantly negatively correlated with transpiration rate and net photosynthetic rate;Leaf area was significantly positively correlated with dry leaf weight.Principal component analysis was used to transform the 8 indicators into 3 independent comprehensive indicators.Transpiration rate and net photosynthetic rate could be used as evaluation indicators for the identification of photosynthetic performance of foxtail millet.Through cluster analysis,37 foxtail millet varieties were divided into three categories(high photosynthetic efficiency,medium photosynthetic efficiency and low photosynthetic efficiency),and 8 high photosynthetic efficiency varieties,including Nenxuan 22,Datong 25,Gonggu 88,Yangu 18,Jingu 20,Mengfeng 7,Longgu 13 and Gonggu 75,were screened out,which could be used as parent materials for high photosynthetic efficiency breeding.The regression model of foxtail millet photosynthetic performance was established by stepwise regression analysis(D=-1.391 984 196+0.000 619 787 003 4X1+0.051 519 289 48X2+0.674 399 672 2X3+0.013 844 082 338X4+0.058 230 284 78X5+0.003 403 878 541X6+0.051 881 997 92X8).