Difference Analysis of Metabolic Profiles of Upper Leaves from Different Plant Types of Qushou No.1
In order to clarify the differences in yield,quality and metabolomics of flue-cured tobacco under different plant types,with Qushou No.1 as the experimental material,the metabolites of upper leaves at the mature stage of two plant types were analyzed by LC-MS non-targeted metabonomics technique combined with the determination of chemical components and sensory quality.The results showed that compared with umbrella-type tobacco plants,the plant height and effective leaf number of cylinder-type tobacco plants increased,and the maximum leaf area decreased.The overall chemical composition coordination and sensory quality of the cylinder-type tobacco plants were better than those of the umbrella-type tobacco plants.The yield of cylinder-type tobacco plants was 5.2％lower than that of umbrella-type tobacco plants,but the average price and output value increased by 25.0％and 20.4％.Through partial least squares-discriminate analysis(PLS-DA),123 differential metabolites were screened out,including amino acids,organic acids,sugars,flavonoids and alkaloids,in which N-formyl-L-methionine,2-oxoarginine,phenylacetylglutamine,trehalose,3-keto-β-D-galactose,D-mannose,2-phenylethanol,jasmonic acid might be the key metabolites in response to different plant types.The differential metabolites were significantly enriched in the pathways of amino acid metabolism and glucose metabolism.Among them,nicotinic acid and nicotinamide metabolism pathway,alanine,aspartic acid and glutamic acid metabolism pathway,and tryptophan metabolism pathway played important regulating roles in the formation of intrinsic quality under different plant types.
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