Identification and Expression Analysis of EIL Family under Saline-alkali Stress in Capsicum annuum L.
EIL(ethylene-insensitive 3-like)genes play important roles in the transduction involved in the ethylene signaling pathway and development of plants.In order to understand the information of EIL gene family members in Capsicum annuum L.,this study analyzed the physicochemical properties,protein structure,phylogeny,gene architecture,conserved domains,promoter cis-acting elements,the expression patterns of CaEILs genes in different tissue parts and under different abiotic stress conditions using bioinformatics.The expression pattern of CaEILs in pepper leaves under saline-alkali stress was also investigated using real-time fluorescence quantitative PCR(qRT-PCR).The results showed that nine CaEILs of Capsicum annuum L.were distributed on six chromosomes.The number of amino acids,molecular weight and aliphatic index were from 209 to 677,23.77 to 76.07 ku,and 63.10 to 87.75,respectively and they were mainly acid,hydrophilic,and unstable nuclear proteins.Phylogenetic analysis showed that CaEILs were divided into four subfamily groups,nine CaEILs were expressed in different degrees in roots,stems,leaves,buds,and flowers,and low temperature stress,high temperature stress,high salt stress,and drought stress induced the expression of CaEILs,which responded to the above abiotic stresses to different degrees.In addition,qRT-PCR was performed to detect CaEILs expression in pepper leaves under saline-alkali stress,and it was found that with the extension of treatment time,the expression of CaEIL1,CaEIL2,CaEIL4,CaEIL5,and CaEIL8 showed an increasing trend,while the expression of CaEIL3,CaEIL6,CaEIL7,and CaEIL9 showed an decreasing trend.The above results lay a foundation for the study of the functions of CaEILs and their mechanisms of action in response to saline-alkali stress.
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