响应面法优化忍冬桑黄总黄酮提取工艺
Optimization of Extraction Process of Total Flavonoids from Sanghuangporus lonicericola by Response Surface Methodology
杨娟娟 1孙波 2王卓仁 2刘启燕 2赵会长 2周洪英 2常菊花3
作者信息
- 1. 长江大学生命科学学院,湖北荆州 434025;湖北省农业科学院经济作物研究所,湖北武汉 430064
- 2. 湖北省农业科学院经济作物研究所,湖北武汉 430064
- 3. 长江大学生命科学学院,湖北荆州 434025
- 折叠
摘要
为了提高忍冬桑黄总黄酮的提取率,以忍冬桑黄(Sanghuangporus lonicericola)子实体为原料,采用超声波辅助乙醇提取其总黄酮,以料液比、乙醇体积分数、超声时间和超声温度进行单因素试验,通过响应面试验对忍冬桑黄总黄酮提取工艺进行优化.结果表明,忍冬桑黄子实体总黄酮最优提取工艺:料液比1∶60、乙醇体积分数60％、超声时间60 min、超声温度76 ℃.在最佳工艺条件下,忍冬桑黄子实体总黄酮提取率为0.80％,与模型预测值0.83％接近,表明该提取条件合理可行,可以提高忍冬桑黄总黄酮提取率,利于忍冬桑黄产品精深加工及桑黄资源的综合利用.
Abstract
In order to improve the extraction rate of total flavonoids from Sanghuangporus lonicericola,this study used the fruiting body of S.lonicericola as the raw material,and used ultrasound assisted ethanol extraction to extract its total flavonoids.The extraction process was optimized by response surface testing,which was conducted based on single factor tests of liquid-solid ratio,ethanol concentration,ultrasonic time,and ultrasonic temperature.The optimal extraction process of total flavonoids from the fruit bodies of S.lonicericola was as follows:solid-liquid ratio of 1∶60,ethanol concentration of 60％,ultrasonic time of 60 min and ultrasonic temperature of 76 ℃.In the optimal conditions,the extraction rate of total flavonoids from S.lonicericola was 0.80％,which was close to the predicted value of 0.83％of the model,indicating that the optimized process is reasonable and feasible,can improve the extraction rate of total flavonoids from S.lonicericola,and facilitate the deep processing of S.lonicericola products and the comprehensive utilization of Sanghuang resources.
关键词
忍冬桑黄/总黄酮/超声波辅助乙醇提取/响应面试验/提取工艺Key words
Sanghuangporus lonicericola/Total flavonoids/Ultrasonic assisted ethanol extraction/Response surface test/Ectraction process引用本文复制引用
基金项目
国家自然科学基金青年基金(31600010)
湖北省重点研发计划(2021BBA245)
国家蚕桑产业技术体系建设项目武汉试验站项目(CARS-18-SYZ11)
湖北省食用菌产业技术体系建设项目(HBHZDZB-2021-023)
出版年
2024
国家科技期刊平台
NSTL
万方数据