The Mitochondrial Genome Sequencing and Analysis of Nysius inconspicuus
To explore the mitochondrial genome structure of Nysius inconspicuus and the phylogeny of Lygaeidae,we newly sequenced the complete mitochondrial genome of N.inconspicuus using next-generation sequencing methods.In phylogenetic analyses,we selected 14 representative species of Lygaeidae as ingroups.One species of Geocoridae was used as outgroup.We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods.The results indicated that the mitochondrial genome of N.inconspicuus encompassed 37 genes(13 protein-coding genes,22 transfer RNA genes,and 2 ribosomal RNA genes)along with 1 non-coding control region,spanning a total length of 14 893 bp(GenBank accession number:OR099699).In the mitochondrial genome of N.inconspicuus,the start codons of protein-coding genes were TTG for cox1 and nad6,AAC for nad4l,while the remaining protein-coding genes began with ATN.As for the stop codons,cox1,cox2,nad3,and nad1 ended with an incomplete T,whereas the rest of protein-coding genes terminated with TAA.In addition,based on three different data matrices,we employed two phylogenetic analysis methods,resulting in largely consistent phylogenetic trees.
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