Cloning and Functional Analysis of Phosphate Transporter SlPT2 Promoter from Solanum lycopersicum
To study the function and activity of the tomato(Solanum lycopersicum)phosphate transporter SlPT2 promoter,tomato variety of Micro-Tom was used as experimental materials,the real-time quantitative PCR was performed to determine the expression pattern of SlPT2 gene,and the sequence of SlPT2 promoter(p SlPT2)was cloned using PCR technology.The cis-acting elements inside p SlPT2 were analyzed through the online promoter analysis websites Plant Care and New Place.The plant expression vector p1300GN-p SlPT2 was constructed using homologous recombination method and transformed into Arabidopsis.The expression patterns and activities of p SlPT2 were studied by using GUS histochemical staining.The real-time quantitative PCR results showed that SlPT2 was expressed mainly in tomato roots;Plant Care and New Place analysis showed that p SlPT2(1 801 bp)not only contained the core promoter elements such as CAAT-Box and TATA-Box,but also contained root-specific expression elements(ROOTMOTIFTAPOX1,RAV1AAT,OSE1ROOTNODULE),light response elements(Box 4,G-Box,TCT-motif),and CGTCA-motif,ABRE,O2-site involved in methyl jasmonate reaction,abscisic acid reaction,regulation of zein metabolism respectively.GUS histochemical staining showed that the SlPT2 promoter drove GUS gene expression in roots of Arabidopsis specifically,which indicated that SlPT2 promoter was a root specific promoter.
Solanum lycopersicumPhosphate transporterTissue specific promoterGUS histochemical stainingCis-acting element
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