Identification and Expression Analysis of HD-ZIP Family in Salvia miltiorrhiza
To screen HD-ZIP genes(SmHD-ZIPs)in Salvia miltiorrhiza in response to high temperature stress,SmHD-ZIPs were identified and analyzed by bioinformatics,and the expression pattern of SmHD-ZIPs under high temperature stress was detected by qPCR.The results showed that there were 44 HD-ZIP genes in S.miltiorrhiza,most of which were unevenly distributed on 8 chromosomes.SmHD-ZIPs could be divided into four subfamilies:HD-ZIPⅠ,HD-ZIPⅡ,HD-ZIPⅢ,HD-ZIPⅣ.The sequence analysis results showed that the amino acid residues of these proteins ranged from 180 to 982,and the relative molecular weight of these proteins ranged from 20.947 ku to 109.620 ku.SmHD-ZIPs were hydrophilic proteins without transmembrane domain,and most of them did not contain signal peptides.The isoelectric points of SmHD-ZIPs were between 4.48 and 10.91,and they were almost expressed in the nucleus with the poor protein stability.Among the 44 SmHD-ZIP genes,there were 10 gene duplication events,all of which were purified selection.The results of structure and motif analysis showed that the exon number of members of the same subfamily was basically the same,and the members of HD-ZIP Ⅲ subfamily had the largest exon number and the largest motif number.Motifs 1 and 6 were conserved motifs of SmHD-ZIP,motifs 10,11,12,13 and 15 was unique to the HD-ZIP Ⅲ subfamily,while motif 5 were unique to the HD-ZIP IV subfamily.By analyzing the expression patterns of SmHD-ZIPs under high temperature(37℃)stress,it was found that the number of genes with increased and decreased expression was basically the same.Among the genes with up-regulated expression,the expression levels of SmHD-ZIP1.11,SmHD-ZIP1.13,SmHD-ZIP2.2,SmHD-ZIP2.5,SmHD-ZIP3.1,SmHD-ZIP3.4,SmHD-ZIP4.9,SmHD-ZIP4.10 and SmHD-ZIP4.12 were increased by more than 10 times,which could be used as candidate gene resources to improve the heat resistance of S.miltiorrhiza.
Salvia miltiorrhizaHD-ZIP familyPhylogenetic treeQuantitative PCRHigh temperature stress
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