Major QTL Mapping and Effect Analysis for Resistance to Southern Corn Rust
Southern corn rust(SCR)is a major maize disease worldwide.In this study,a BC1F4population including 165 families was derived from the cross between the susceptible line Lx9801 and the resistant line TY4,which was used for dissecting the QTLs for resistance to SCR.A 23K-SNP chip was used for genotyping the family lines of the BC1F4 population as well as the parental lines.In total 4 654 polymorphic SNP markers between the parental lines were selected to construct the high-density genetic linkage map.Combined with the phenotype evaluation of resistance to SCR in three environments,a total of six QTLs against SCR were detected,which could explain 3.93%—17.87%of the phenotypic variation.Among these QTLs,one QTL located on chromosome 6(i.e.,qSCR6.01)was a stable QTL in all three environments,which could explain up to 17.87%of the phenotypic variation.The BC1F5 population including 366 families was used for fine-mapping qSCR6.01.Combined with marker development in the QTL region and resistance identification of key recombinant plants,the QTL was finally narrowed down to the interval of 4.09 Mb between the markers M3 and M4,and temporarily named as RppT.
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