Establishment of Indirect ELISA Antibody Detection Method Based on Penton Protein of Fowl Adenovirus Serotype 4
Penton protein is the main structural protein that constitutes the capsid of fowl adenovirus serotype 4(FAdV-4),which has high conservation and good immunogenicity.An indirect ELISA antibody detection method based on the purified Penton protein of FAdV-4 was established.The results showed that the optimal coating concentration of Penton protein was 2 µg/mL,the serum dilution multiple was 200 times,the enzyme-labeled antibody dilution multiple was 1∶5 000,the positive critical value was 0.222,and the sensitivity could reach 1∶6 400.There was no cross-reaction with infectious bronchitis virus,infectious bursal disease virus,and infectious laryngotracheitis virus,and the specificity was good.The coefficient of variation of inter-batch and intra-batch repeatability was less than 10%.The positive detection rate in 100 clinical serum samples was 77%,and the coincidence rate with the commercial kit was 98%.In summary,the ELISA antibody detection method established in this study can be used for the clinical detection of antibodies against FAdV-4.
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