Fungal Diversity in Rotten Bulb and Cultivated Soil of Lilium lancifolium
To understand the fungal communities in rotten bulb and cultivated soil of Lilium lancifolium,as well as to explore the mechanism of bulb rot disease occurrence,the cultivable fungi and pathogenic isolates were identified through isolation and cultivation methods.The differences in fungal community between diseased and healthy soil were compared by using Illumina MiSeq high-throughput sequencing.273 strains were isolated and identified from rotten bulb of Lilium lancifolium,with the dominant genus being Fusarium.Among them,eight genera and 14 species of fungi were found to be pathogenic to Lilium lancifolium bulb.Notably,the relative abundance of Alternaria,Botrytis,Fusarium,Penicillium,and Trametes was higher in diseased soil compared to healthy soil by 0.025,0.006,0.132,0.078,and 0.002 percent points,respectively.Moreover,fungal α-diversity was higher in diseased soil,and β-diversity analysis confirmed distinct fungal community structures between diseased and healthy soils.Lefse analysis revealed that Cephaliophora,Candida,Chaetomium strumarium,etc.were markers of diseased soil,whereas Leythophora and its species indicated healthy soil.Our findings suggest that the accumulation of pathogenic fungi in the soil,along with shifts in fungal diversity and community structure,might be the key reasons for the development of bulb rot disease in Lilium lancifolium.
Lilium lancifoliumBulb rotPathogenFungal community structureSoil health
国家科技期刊平台
NSTL
万方数据
