Identification and Evaluation of Resistance to Stripe Rust of 480 Wheat Germplasms
The main purpose of the present study is to understand the resistance to stripe rust and the utilization of stripe rust resistant genes in current bred wheat varieties,and find the varieties containing novel stripe rust resistant loci.Totally,480 domestic and foreign wheat germplasms were collected from various planting regions.The varieties were inoculated with mixed races of CYR32,CYR33 and CYR34 for testing the adult plant resistance.Simultaneously,the resistance to leaf rust and powdery mildew was investigated in infected field.The KASP markers closely linked or co-segregated with stripe rust,leaf rust and powdery mildew genes,Yr18/Lr34/Sr57/Pm38(7DS),Yr29/Lr46/Pm39/Sr58(1BL)and Yr30/Lr27/Sr2/Sb3(3BS),were selected for identifying the previous reported pleiotropic resistant genes.The results showed that there were 35(accounting for 7.29%)germplasms showed immunization to CYR32,CYR33 and CYR34,while the numbers of high-resistant and medium-resistant germplasms were 69(14.38%)and 79(16.46%),respectively.And the other 297(61.88%)germplasms demonstrated medium-or high-susceptible to the inoculated races.There were 13,10 and 7 germplasms containing the Yr18,and Yr29 and Yr30 genes,respectively;only 3 germplasms contained two of the resistant genes;no germplasm was detected with all the three resistant genes.In conclusion,183(accounting for 38.12%)germplasms showed resistant to stripe rust at adult plant stage,and 27 germplasms were detected with the above resistant genes.Yr18 or Yr30 gene showed resistant to the current races of stripe rust,however,the resistance level of Yr29 gene was partly lost at adult plant stage.
万方数据
维普
